PathPlus(tm) NR5A2 / LRH-1 Antibody (Internal)

LifeSpan BioSciences

PathPlus(tm) NR5A2 / LRH-1 Antibody (Internal)

23

Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA.. IHC, IHC-P (10 - 20 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

NR5A2

nuclear receptor subfamily 5 group A member 2

B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2, nuclear receptor subfamily 5 group A member 2, CYP7A promoter-binding factor, b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus, fetoprotein-alpha 1 (AFP) transcription factor, hepatocytic transcription factor hB1F-3

Rabbit

Canine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Rat, Xenopus, Zebra Fish

-20°C or -80°C,2°C to 8°C

41116161