PathPlus(tm) NR5A2 / LRH-1 Antibody (Internal)
LS-A2453
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Rat, Xenopus, Zebra Fish
TargetNR5A2
Overview
- SupplierLifeSpan BioSciences
- Product NamePathPlus(tm) NR5A2 / LRH-1 Antibody (Internal)
- Delivery Days Customer23
- Antibody SpecificityHuman NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Application Supplier NoteImmunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA.. IHC, IHC-P (10 - 20 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA.
- ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2494
- Target nameNR5A2
- Target descriptionnuclear receptor subfamily 5 group A member 2
- Target synonymsb1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus; B1F; B1F2; CPF; CYP7A promoter-binding factor; fetoprotein-alpha 1 (AFP) transcription factor; FTF; FTZ-F1; FTZ-F1beta; hB1F-2; hepatocytic transcription factor hB1F-3; liver nuclear receptor homolog-1 variant 2; LRH1; LRH-1; nuclear receptor subfamily 5 group A member 2
- HostRabbit
- ReactivityCanine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Rat, Xenopus, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203