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PathPlus(tm) NR5A2 / LRH-1 Antibody (Internal)

Research Use Only
LS-A2453
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Rat, Xenopus, Zebra Fish
TargetNR5A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) NR5A2 / LRH-1 Antibody (Internal)
  • Delivery Days Customer
    23
  • Antibody Specificity
    Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
  • Application Supplier Note
    Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA.. IHC, IHC-P (10 - 20 µg/ml) Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 2.5 - 5 ug/ml. Antibody does not work with tissues fixed in 4% PFA.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2494
  • Target name
    NR5A2
  • Target description
    nuclear receptor subfamily 5 group A member 2
  • Target synonyms
    b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus; B1F; B1F2; CPF; CYP7A promoter-binding factor; fetoprotein-alpha 1 (AFP) transcription factor; FTF; FTZ-F1; FTZ-F1beta; hB1F-2; hepatocytic transcription factor hB1F-3; liver nuclear receptor homolog-1 variant 2; LRH1; LRH-1; nuclear receptor subfamily 5 group A member 2
  • Host
    Rabbit
  • Reactivity
    Canine, Chicken, Equine, Hamster, Human, Monkey, Mouse, Rat, Xenopus, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203