PathPlus(tm) PDPN / Podoplanin Antibody (Concentrated)

LifeSpan BioSciences

PathPlus(tm) PDPN / Podoplanin Antibody (Concentrated)

23

Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.. ELISA (1:100 - 1:200), IHC-P (1:100) Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.

ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

Unconjugated

...

PDPN

podoplanin

AGGRUS; glycoprotein 36; glycoprotein, 36-KD; GP36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS8; PA2.26; PA2.26 antigen; podoplanin; T1A; T1A2; T1A-2; T1-alpha; TI1A

Mouse

IgG2a

Human

2°C to 8°C

12352203