PathPlus(tm) RPE65 Antibody (clone 401.8B11.3D9)
LS-B90
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat
TargetRPE65
Overview
- SupplierLifeSpan BioSciences
- Product NamePathPlus(tm) RPE65 Antibody (clone 401.8B11.3D9)
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B90 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B90 was determined to be 2.5 ug/ml.. Flo, ICC, IHC-P (2.5 - 5 µg/ml), WB (1:5000 - 1:10000) Immunohistochemistry: LS-B90 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B90 was determined to be 2.5 ug/ml.
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID401.8B11.3D9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6121
- Target nameRPE65
- Target descriptionretinoid isomerohydrolase RPE65
- Target synonymsBCO3, LCA2, RP20, mRPE65, p63, rd12, sRPE65, retinoid isomerohydrolase, BCO family, member 3, RBP-binding membrane protein, RPE65, retinoid isomerohydrolase, all-trans-retinyl-palmitate hydrolase, lutein isomerase, meso-zeaxanthin isomerase, retinal pigment epithelium specific protein 65, retinal pigment epithelium-specific 65 kDa protein, retinal pigment epithelium-specific protein 65kDa, retinitis pigmentosa 20 (autosomal recessive), retinol isomerase
- HostMouse
- IsotypeIgG1
- ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C or -80°C
- UNSPSC41116161

![RPE65 antibody [N1C3] detects RPE65 protein at retinal pigment epithelium by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: RPE65 stained by RPE65 antibody [N1C3] (GTX103472) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuronal marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX103472/GTX103472_44279_20210625_IHC-P_M_w_23060119_842.webp)



