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ProductsBack/PathPlus(tm) SMARCA4 / BRG1 Antibody (aa75-125)

PathPlus(tm) SMARCA4 / BRG1 Antibody (aa75-125)

LS-B11769
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSMARCA4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) SMARCA4 / BRG1 Antibody (aa75-125)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), IP (2 - 10 µg/mg lysate), WB (1:2000 - 1:10000) IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6597
  • Target name
    SMARCA4
  • Target description
    SWI/SNF related BAF chromatin remodeling complex subunit ATPase 4
  • Target synonyms
    BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A, BRM/SWI2-related gene 1, SNF2-like 4, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4, brahma protein-like 1, global transcription activator homologous sequence, homeotic gene regulator, mitotic growth and transcription activator, nuclear protein GRB1, protein BRG-1, protein brahma homolog 1, sucrose nonfermenting-like 4, transcription activator BRG1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of BRG1 using anti-BRG1 antibody (A00223-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human 22RV1 whole cell lysates, Lane 8: rat small intestine tissue lysates, Lane 9: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRG1 antigen affinity purified polyclonal antibody (Catalog # A00223-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRG1 at approximately 220KD. The expected band size for BRG1 is at 185KD.
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