PathPlus(tm) TH / Tyrosine Hydroxylase Antibody

LifeSpan BioSciences

PathPlus(tm) TH / Tyrosine Hydroxylase Antibody

23

Immunohistochemistry: LS-B3443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Western Blot: 1:1000 on 10 ug of PC12 lysates. Immunofluorescence: 1:100-1:1000. Fixed with 4% PFA overnight at 4C.. ELISA, IF (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (1:100), IP, WB (1:1000) Immunohistochemistry: LS-B3443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Western Blot: 1:1000 on 10 ug of PC12 lysates. Immunofluorescence: 1:100-1:1000. Fixed with 4% PFA overnight at 4¦C.

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

TH

tyrosine hydroxylase

DYT14, DYT5b, TYH, tyrosine 3-monooxygenase, dystonia 14, tyrosine 3-hydroxylase

Rabbit

IgG

Feline, Ferret, Human, Mouse, Rat

-20°C,2°C to 8°C

41116161