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ProductsBack/PATZ1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PATZ1 (Cat# RC205802, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PATZ1 (Cat# TA809258)(1:2000). Positive lysates [LY410365] (100ug) and [LC410365] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PATZ1 (Cat# RC205802, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PATZ1 (Cat# TA809258)(1:2000). Positive lysates [LY410365] (100ug) and [LC410365] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PATZ1 (Cat# RC205802, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PATZ1 (Cat# TA809258)(1:2000). Positive lysates [LY410365] (100ug) and [LC410365] (20ug) can be purchased separately from OriGene.

PATZ1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D2]

TA809258BM
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPATZ1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PATZ1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D2]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2D2
  • Gene ID23598
  • Target name
    PATZ1
  • Target description
    POZ/BTB and AT hook containing zinc finger 1
  • Target synonyms
    MAZR, PATZ, RIAZ, ZBTB19, ZNF278, ZSG, dJ400N23, POZ-, AT hook-, and zinc finger-containing protein 1, BTB-POZ domain zinc finger transcription factor, MAZ-related factor, POZ-AT hook-zinc finger protein, protein kinase A RI subunit alpha-associated protein, zinc finger and BTB domain-containing protein 19, zinc finger protein 278, zinc finger sarcoma gene protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9HBE1
  • Protein Name
    POZ-, AT hook-, and zinc finger-containing protein 1
  • Scientific Description
    PATZ1 mouse monoclonal antibody,clone OTI2D2, HRP conjugated
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PATZ1 using anti-PATZ1 antibody (A06823-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PATZ1 antigen affinity purified polyclonal antibody (Catalog # A06823-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PATZ1 at approximately 70 kDa. The expected band size for PATZ1 is at 74 kDa.
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