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Western Blot analysis of 293 cells using PAR4 Polyclonal Antibody
Western Blot analysis of 293 cells using PAR4 Polyclonal Antibody
Western Blot analysis of 293 cells using PAR4 Polyclonal Antibody

PAWR Antibody

CSB-PA003704
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPAWR
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAWR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5074
  • Target name
    PAWR
  • Target description
    pro-apoptotic WT1 regulator
  • Target synonyms
    PAR4; Par-4; PRKC apoptosis WT1 regulator protein; PRKC, apoptosis, WT1, regulator; prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4; prostate apoptosis response-4; transcriptional repressor PAR4; WT1-interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96IZ0
  • Protein Name
    PRKC apoptosis WT1 regulator protein
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAWR using anti-PAWR antibody (A03637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAWR antigen affinity purified polyclonal antibody (Catalog # A03637-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAWR at approximately 41 kDa. The expected band size for PAWR is at 37 kDa.
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