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PAWR / PAR4 Antibody

LS-C402504
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPAWR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PAWR / PAR4 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:25 - 1:100), WB (1:1000 - 1:3000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5074
  • Target name
    PAWR
  • Target description
    pro-apoptotic WT1 regulator
  • Target synonyms
    PAR4, Par-4, PRKC apoptosis WT1 regulator protein, PRKC, apoptosis, WT1, regulator, WT1-interacting protein, prostate apoptosis response protein 4, prostate apoptosis response protein PAR-4, prostate apoptosis response-4, transcriptional repressor PAR4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAWR using anti-PAWR antibody (A03637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAWR antigen affinity purified polyclonal antibody (Catalog # A03637-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAWR at approximately 41 kDa. The expected band size for PAWR is at 37 kDa.
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