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Western blot analysis of PAX1 Antibody (C-term) in HepG2, MDA-MB453, 293, K562, MCF-7 cell line lysates (35ug/lane). This demonstrates the PAX1 antibody detected the PAX1 protein (arrow).
Western blot analysis of PAX1 Antibody (C-term) in HepG2, MDA-MB453, 293, K562, MCF-7 cell line lysates (35ug/lane). This demonstrates the PAX1 antibody detected the PAX1 protein (arrow).
Western blot analysis of PAX1 Antibody (C-term) in HepG2, MDA-MB453, 293, K562, MCF-7 cell line lysates (35ug/lane). This demonstrates the PAX1 antibody detected the PAX1 protein (arrow).

PAX1 (C-term) Rabbit Polyclonal Antibody

AP53177PU-N
OriGene
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetPAX1
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PAX1 (C-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5075
  • Target name
    PAX1
  • Target description
    paired box 1
  • Target synonyms
    HUP48, OFC2, OTFCS2, paired box protein Pax-1, paired box gene 1, paired domain gene HuP48
  • Host
    Rabbit
  • Protein IDP15863
  • Protein Name
    Paired box protein Pax-1
  • Scientific Description
    PAX1 (C-term) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAX1 using anti-PAX1 antibody (A04559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Sw579 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat ovary tissue lytsates, Lane 7: rat lung tissue lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse ovary tissue lysates, Lane 10: mouse lung tissue lysates, Lane 11: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX1 antigen affinity purified polyclonal antibody (Catalog # A04559-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX1 at approximately 60KD. The expected band size for PAX1 is at 60KD.
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