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IHC-P analysis of rat lymphaden tissue using GTX04784 PAX2 antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 1 microg/mL
IHC-P analysis of rat lymphaden tissue using GTX04784 PAX2 antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 1 microg/mL
IHC-P analysis of rat lymphaden tissue using GTX04784 PAX2 antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 1 microg/mL

PAX2 antibody

GTX04784
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPAX2
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Overview

  • Supplier
    GeneTex
  • Product Name
    PAX2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5 microg/mL. IHC-P: 0.5-1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5076
  • Target name
    PAX2
  • Target description
    paired box 2
  • Target synonyms
    FSGS7; paired box homeotic gene 2; paired box protein Pax-2; PAPRS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02962
  • Protein Name
    Paired box protein Pax-2
  • Scientific Description
    PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor suppressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Pax2 using anti-Pax2 antibody (PB9734). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Lung Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pax2 antigen affinity purified polyclonal antibody (Catalog # PB9734) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pax2 at approximately 45 kDa. The expected band size for Pax2 is at 45 kDa.
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