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Western Blot analysis of 293 cells using Pax-5 Polyclonal Antibody
Western Blot analysis of 293 cells using Pax-5 Polyclonal Antibody
Western Blot analysis of 293 cells using Pax-5 Polyclonal Antibody

PAX5 Antibody

CSB-PA003710
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetPAX5
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAX5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5079
  • Target name
    PAX5
  • Target description
    paired box 5
  • Target synonyms
    ALL3, BSAP, PAX-5, paired box protein Pax-5, B-cell lineage specific activator, paired box homeotic gene 5, paired domain gene 5, transcription factor PAX 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02548
  • Protein Name
    Paired box protein Pax-5
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAX5 using anti-PAX5 antibody (A00669-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX5 antigen affinity purified polyclonal antibody (Catalog # A00669-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX5 at approximately 45 kDa. The expected band size for PAX5 is at 42 kDa.
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