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Anti-PAX5 Antibody

AMAB91963
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPAX5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PAX5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL14548
  • Conjugate
    Unconjugated
  • Gene ID5079
  • Target name
    PAX5
  • Target description
    paired box 5
  • Target synonyms
    ALL3; B-cell lineage specific activator; BSAP; paired box homeotic gene 5; paired box protein Pax-5; paired domain gene 5; transcription factor PAX 5
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ02548
  • Protein Name
    Paired box protein Pax-5
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of PAX5 using anti-PAX5 antibody (A00669-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX5 antigen affinity purified polyclonal antibody (Catalog # A00669-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX5 at approximately 45 kDa. The expected band size for PAX5 is at 42 kDa.
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