IP analysis of Jurkat cell lysate using GTX12188 PCAF antibody. Lane 1 : 5 microg/mL antibody Lane 2 : Negative control: without antibody Lane 3 : 10 microg/mL antibody Lane 4 : Negative control: without cell extract
![HeLa whole cell and nuclear extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX12188) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX12188/GTX12188_30919_20161117_WB_Fraction_w_23060519_791.webp "HeLa whole cell and nuclear extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX12188) diluted at 1:1000.")
IP analysis of Jurkat cell lysate using GTX12188 PCAF antibody. Lane 1 : 5 microg/mL antibody Lane 2 : Negative control: without antibody Lane 3 : 10 microg/mL antibody Lane 4 : Negative control: without cell extract
PCAF antibody
GTX12188
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityCanine, Human
TargetKAT2B
Overview
- SupplierGeneTex
- Product NamePCAF antibody - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityPCAF (p300/CBP associated factor, P/CAF), a type A histone acetyltransferases (HAT), has been identified in several species. Human PCAF is a ubiquitous protein member of the GCN5-related N-acetyltransferase (GNAT) superfamily. It interacts with the p300 a
- Application Supplier NoteWB: 1:500. IP: 5-10 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID8850
- Target nameKAT2B
- Target descriptionlysine acetyltransferase 2B
- Target synonymsCAF; CREBBP-associated factor; histone acetylase PCAF; histone acetyltransferase KAT2B; histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; P/CAF; p300/CBP-associated factor; PCAF; spermidine acetyltransferase KAT2B
- HostRabbit
- IsotypeIgG
- Protein IDQ92831
- Protein NameHistone acetyltransferase KAT2B
- Scientific DescriptionCBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
- ReactivityCanine, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203