More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PCCA Polyclonal Antibody
Western blot analysis of extracts of various cell lines using PCCA Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using PCCA Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using PCCA Polyclonal Antibody at dilution of 1:3000.

PCCA Polyclonal Antibody

E-AB-63961
Elabscience
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Elabscience
  • Product Name
    PCCA Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
PCCA AntibodyCAB9486
ReactivityMouse
TargetPCCA
  • SizePrice
Product group Antibodies
Biomatik
PCCA Polyclonal AntibodyCAC12823
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetPCCA
  • SizePrice
Anti-PCCA Antibody
Product group Antibodies
Antibodies.com
Anti-PCCA AntibodyA16943
ApplicationsWestern Blot
ReactivityMouse, Rat
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PCCA Antibody144-09486
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPCCA
  • SizePrice
Product group Antibodies
References
GeneTex
PCCA antibodyGTX114618
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetPCCA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PCCA AntibodyLS-C411020
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPCCA
  • SizePrice
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-PCCA AntibodyHPA041716
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetPCCA
  • SizePrice
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, Hela whole cell lysate All lanes: PCCA antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 78, 76 kDa Observed band size: 81 kDa
Product group Antibodies
Cusabio
PCCA AntibodyCSB-PA017522LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPCCA
  • SizePrice
Figure 1. Western blot analysis of PCCA using anti-PCCA antibody (A03395-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCCA antigen affinity purified polyclonal antibody (Catalog # A03395-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCCA at approximately 70 kDa. The expected band size for PCCA is at 80 kDa.
Product group Antibodies
Boster Bio
Anti-PCCA Antibody Picoband(r)A03395-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPCCA
  • SizePrice