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Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: PCCB antibody at 7.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 61 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: PCCB antibody at 7.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 61 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: PCCB antibody at 7.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59, 61 kDa Observed band size: 59 kDa

PCCB Antibody

CSB-PA017523LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPCCB
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Overview

  • Supplier
    Cusabio
  • Product Name
    PCCB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5096
  • Target name
    PCCB
  • Target description
    propionyl-CoA carboxylase subunit beta
  • Target synonyms
    PCCase subunit beta; propanoyl-CoA:carbon dioxide ligase subunit beta; propionyl CoA carboxylase, beta polypeptide; propionyl Coenzyme A carboxylase, beta polypeptide; propionyl-CoA carboxylase beta chain, mitochondrial; propionyl-CoA carboxylase beta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05166
  • Protein Name
    Propionyl-CoA carboxylase beta chain, mitochondrial
  • Scientific Description
    cytosol, mitochondrial matrix, mitochondrion, CoA carboxylase activity, propionyl-CoA carboxylase activity, biotin metabolic process, fatty acid catabolic process, short-chain fatty acid catabolic process
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PCCB using anti-PCCB antibody (A04326-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCCB antigen affinity purified polyclonal antibody (Catalog # A04326-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCCB at approximately 55 kDa. The expected band size for PCCB is at 58 kDa.
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