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PCDH15 Antibody (aa112-161)

LS-C81525
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPCDH15
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PCDH15 Antibody (aa112-161)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID65217
  • Target name
    PCDH15
  • Target description
    protocadherin related 15
  • Target synonyms
    cadherin-related family member 15; CDHR15; DFNB23; protocadherin-15; USH1F
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCDH15 using anti-PCDH15 antibody (A03591-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate, Lane 2: human U2OS whole cell lysate, Lane 3: human Hela whole cell lysate, Lane 4: rat brain tissue lysate, Lane 5: rat lung tissue lysate, Lane 6: mouse brain tissue lysate, Lane 7: mouse lung tissue lysate, Lane 8: mouse kidney tissue lysate, Lane 9: mouse spleen tissue lysate, Lane 10: mouse Neuro-2a whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDH15 antigen affinity purified polyclonal antibody (Catalog # A03591-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDH15 at approximately 190KD. The expected band size for PCDH15 is at 216KD.
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