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PCDHA8 Antibody

PACO64893
Assay Genie
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPCDHA8
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PCDHA8 Antibody
  • Delivery Days Customer
    9
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID56140
  • Target name
    PCDHA8
  • Target description
    protocadherin alpha 8
  • Target synonyms
    PCDH-ALPHA8, protocadherin alpha-8, KIAA0345-like 6, PCDH-alpha-8
  • Host
    Rabbit
  • Protein IDQ9Y5H6
  • Protein Name
    Protocadherin alpha-8
  • Scientific Description
    PCDHA8 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, IHC, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PCDHA8 using anti-PCDHA8 antibody (A15533). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDHA8 antigen affinity purified polyclonal antibody (Catalog # A15533) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDHA8 at approximately 150 kDa. The expected band size for PCDHA8 is at 103 kDa.
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