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PCDHA8 Antibody

PACO64893
Assay Genie
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPCDHA8
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PCDHA8 Antibody
  • Delivery Days Customer
    9
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID56140
  • Target name
    PCDHA8
  • Target description
    protocadherin alpha 8
  • Target synonyms
    PCDH-ALPHA8, protocadherin alpha-8, KIAA0345-like 6, PCDH-alpha-8
  • Host
    Rabbit
  • Protein IDQ9Y5H6
  • Protein Name
    Protocadherin alpha-8
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -80°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of PCDHA8 using anti-PCDHA8 antibody (A15533). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCDHA8 antigen affinity purified polyclonal antibody (Catalog # A15533) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCDHA8 at approximately 150 kDa. The expected band size for PCDHA8 is at 103 kDa.
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