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PCOLCE Antibody

CAB15298
Assay Genie
ReactivityHuman
Product group Antibodies
TargetPCOLCE
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PCOLCE Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5118
  • Target name
    PCOLCE
  • Target description
    procollagen C-endopeptidase enhancer
  • Target synonyms
    PCPE, PCPE-1, PCPE1, procollagen C-endopeptidase enhancer 1, procollagen C-proteinase enhancer 1, procollagen COOH-terminal proteinase enhancer 1, procollagen, type 1, COOH-terminal proteinase enhancer, type 1 procollagen C-proteinase enhancer protein, type I procollagen COOH-terminal proteinase enhancer
  • Host
    Rabbit
  • Protein IDQ15113
  • Protein Name
    Procollagen C-endopeptidase enhancer 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCOLCE using anti-PCOLCE antibody (A06428-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCOLCE antigen affinity purified polyclonal antibody (Catalog # A06428-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCOLCE at approximately 50 kDa. The expected band size for PCOLCE is at 48 kDa.
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TargetPCOLCE
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Procollagen C-Endopeptidase Enhancer (PCOLCE) Polyclonal AntibodyCAU26154
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
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Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.
Product group Antibodies
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Anti-PCOLCE AntibodyHPA042927
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IHC image of CSB-PA614881LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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TargetPCOLCE
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TargetPCOLCE
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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PCOLCE antibody [N1C3] (GTX104154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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TargetPCOLCE
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