FACS analysis of HeLa cells using GTX50053 PD1 antibody [4F12]. Red : Primary antibody Black : Isotype control Dilution : 2-5microg for 1x10? cells
![ICC/IF analysis of HeLa cells using GTX50053 PD1 antibody [4F12]. Green : Primary antibody Blue : cell nucleus Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX50053/GTX50053_20180517_ICCIF_w_23060823_630.webp "ICC/IF analysis of HeLa cells using GTX50053 PD1 antibody [4F12]. Green : Primary antibody Blue : cell nucleus Dilution : 1:100")
![WB analysis of various cell lysate using GTX50053 PD1 antibody [4F12]. Lane 1 : 293T cell lysate Lane 2 : Raji cell lysate Dilution : 1:3000 Loading : 40microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX50053/GTX50053_20180517_WB_w_23060823_269.webp "WB analysis of various cell lysate using GTX50053 PD1 antibody [4F12]. Lane 1 : 293T cell lysate Lane 2 : Raji cell lysate Dilution : 1:3000 Loading : 40microg")
FACS analysis of HeLa cells using GTX50053 PD1 antibody [4F12]. Red : Primary antibody Black : Isotype control Dilution : 2-5microg for 1x10? cells
PD1 antibody [4F12]
GTX50053
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPDCD1
Overview
- SupplierGeneTex
- Product NamePD1 antibody [4F12]
- Delivery Days Customer9
- Application Supplier NoteWe recommend the following starting dilutions:Western Blot: Use at 1:500 ~ 1,000.Optimal working concentrations should be determined experimentally by the end user.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4F12
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5133
- Target namePDCD1
- Target descriptionprogrammed cell death 1
- Target synonymsCD279; hPD-1; hPD-l; hSLE1; PD1; PD-1; programmed cell death 1 protein; programmed cell death protein 1; protein PD-1; SLEB2; systemic lupus erythematosus susceptibility 2
- HostMouse
- IsotypeIgG2b
- Protein IDQ15116
- Protein NameProgrammed cell death protein 1
- Scientific DescriptionThis gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203