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Anti-PD-1 [5C4.B8 (Nivolumab)]

Ab00791-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetPDCD1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-PD-1 [5C4.B8 (Nivolumab)]
  • Delivery Days Customer
    7
  • Application Supplier Note
    Nivolumab has been shown to bind to PD-1-expressing CHO cells (EC50 ~1.66nM). Nivolumab binds CD4+ T cells (EC50 ~0.64 nM) and stains only memory and effector, and not naiive CD4+ or CD8+ T cells from human peripheral blood by FC. The antibody is able to block the interaction between PD-1 and its ligands PDL-1 and PDL-2 (IC50 ~2.52 nM and ~2.59 nM, respectively - determined by SPR) - these IC50 values are also similar to that measured by FACS to evaluate ligand binding to PD-1 expressed on CHO cells. In an allogenic T-cell/DC MLR, Nivolumab-mediated inhibition of PD-1 results in enhancement of IFNgamma release, and also enhances IL-2 secretion (97-139% over an isotype control) in response to the superantigen SEB using human peripheral blood mononuclear cells. The same is also observed in a CMV-restimulation assay. Nivolumab at very low concentrations (~1.5 ng/mL) is able to enhance T-cell reactivity in the presence of a T-cell receptor stimulus - nivolumab has no stimulatory effect in the absence of antigen or T-cell receptor stimulus. In the therapeutically used human IgG4 (S228P) format, this antibody is unable to mediate ADCC (antibody-dependent cell-mediated cytotoxicity) or CDC (complement-dependent cytotoxicity).
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
  • Applications Supplier
    IHC; FC; SPR; block
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C4.B8 (Nivolumab)
  • Gene ID5133
  • Target name
    PDCD1
  • Target description
    programmed cell death 1
  • Target synonyms
    CD279; hPD-1; hPD-l; hSLE1; PD1; PD-1; programmed cell death 1 protein; programmed cell death protein 1; protein PD-1; SLEB2; systemic lupus erythematosus susceptibility 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15116
  • Protein Name
    Programmed cell death protein 1
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original human IgG4 (S228P) format, for improved compatibility with existing reagents, assays and techniques. NOT FOR THERAPEUTIC USE- This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human, cynomolgus
  • Reactivity Supplier Note
    mAb PD1.5 was prepared by immunizing IgH and IgK knock-out transgenic mice possessing a human immunoglobulin (heavy chain) minilocus with recombinant human PD-1-Fc protein consisting of the extracellular domain of PD-1 (amino acids 1–167) and the Fc portion of human IgG1, and Chinese hamster ovary (CHO) cells expressing human PD-1. Nivolumab was generated by grafting the variable regions of PD1.5 onto human kappa and IgG4 constant regions containing an S228P mutation (prevents Fab arm exchange with endogenous IgG4 antibodies).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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