Anti-PD-1 [5C4.B8 (Nivolumab)]

Absolute Antibody

Anti-PD-1 [5C4.B8 (Nivolumab)]

7

ImmunoHistoChemistry

IHC; FC; SPR; block

Research Use Only

5C4.B8 (Nivolumab)

Human

IgG1

Human

Human, cynomolgus

mAb PD1.5 was prepared by immunizing IgH and IgK knock-out transgenic mice possessing a human immunoglobulin (heavy chain) minilocus with recombinant human PD-1-Fc protein consisting of the extracellular domain of PD-1 (amino acids 1–167) and the Fc portion of human IgG1, and Chinese hamster ovary (CHO) cells expressing human PD-1. Nivolumab was generated by grafting the variable regions of PD1.5 onto human kappa and IgG4 constant regions containing an S228P mutation (prevents Fab arm exchange with endogenous IgG4 antibodies).

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

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