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Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PDCD1LG2 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PDCD1LG2 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PDCD1LG2 Polyclonal Antibody at dilution 1:50

PDCD1LG2 Polyclonal Antibody

E-AB-14781
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PDCD1LG2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    Programmed cell death 1 ligand 2(also known as PD-L2, B7-DC) is aproteinthat in humans is encoded by thePDCD1LG2gene.PDCD1LG2 has also been designated asCD273(cluster of differentiation273). Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of B7 DC using anti-B7 DC antibody (PB10010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B7 DC antigen affinity purified polyclonal antibody (Catalog # PB10010) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B7 DC at approximately 37 kDa. The expected band size for B7 DC is at 31 kDa.
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