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Cell lysates of HepG2(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of HepG2(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of HepG2(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

PDCD4 antibody [k4C1]

GTX50048
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetPDCD4
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Overview

  • Supplier
    GeneTex
  • Product Name
    PDCD4 antibody [k4C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend the following starting dilutions:Western Blot: Use at 1:1,000~ 3,000.Optimal working concentrations should be determined experimentally by the end user.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    k4C1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27250
  • Target name
    PDCD4
  • Target description
    programmed cell death 4
  • Target synonyms
    H731; neoplastic transformation inhibitor protein; nuclear antigen H731; programmed cell death 4 (neoplastic transformation inhibitor); programmed cell death protein 4; protein 197/15a
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ53EL6
  • Protein Name
    Programmed cell death protein 4
  • Scientific Description
    This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.
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