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IHC-P analysis of endometrium tissue using GTX83931 PDE10A antibody [1B2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of endometrium tissue using GTX83931 PDE10A antibody [1B2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of endometrium tissue using GTX83931 PDE10A antibody [1B2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50

PDE10A antibody [1B2]

GTX83931
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPDE10A
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Overview

  • Supplier
    GeneTex
  • Product Name
    PDE10A antibody [1B2] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1B2
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10846
  • Target name
    PDE10A
  • Target description
    phosphodiesterase 10A
  • Target synonyms
    3',5' cAMP/cGMP phosphodiesterase; ADSD2; cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; dJ416F21.1 (phosphodiesterase 10A); HSPDE10A; IOLOD; LINC00473; PDE10A19; phosphodiesterase 10A1 (PDE10A1); phosphodiesterase 10A7 (PDE10A7)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y233
  • Protein Name
    cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
  • Scientific Description
    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDE10A using anti-PDE10A antibody (A02605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE10A antigen affinity purified polyclonal antibody (Catalog # A02605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE10A at approximately 100KD. The expected band size for PDE10A is at 100KD.
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