Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX118886/GTX118886_42214_20180323_WB_B_w_23060519_781.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX118886/GTX118886_42340_20180323_WB_M_R_w_23060519_181.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000 and competitor's antibody (ab177933) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX118886/GTX118886_42340_20180323_WB_competitor_watermark_w_23060519_445.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000 and competitor's antibody (ab177933) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX118886/GTX118886_45063_20230602_WB_TPM_watermark_24082301_422.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term (GTX118886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PDE10A antibody [C2C3], C-term
GTX118886
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDE10A
Overview
- SupplierGeneTex
- Product NamePDE10A antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.1 mg/ml
- ConjugateUnconjugated
- Gene ID10846
- Target namePDE10A
- Target descriptionphosphodiesterase 10A
- Target synonymsADSD2, HSPDE10A, IOLOD, LINC00473, PDE10A19, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, 3',5' cAMP/cGMP phosphodiesterase, dJ416F21.1 (phosphodiesterase 10A), phosphodiesterase 10A1 (PDE10A1), phosphodiesterase 10A7 (PDE10A7)
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y233
- Protein NamecAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
- Scientific DescriptionVarious cellular responses are regulated by the second messengers cAMP and cGMP. Phosphodiesterases, such as PDE10A, eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate (Fujishige et al., 2000 [PubMed 10998054]).[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![WB analysis of various samples using GTX83931 PDE10A antibody [1B2]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83931/GTX83931_3477_WB_w_23061420_907.webp)
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