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PDE10A Antibody (clone OTI4A10)

LS-C172334
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetPDE10A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PDE10A Antibody (clone OTI4A10)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    Flo, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4A10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10846
  • Target name
    PDE10A
  • Target description
    phosphodiesterase 10A
  • Target synonyms
    3',5' cAMP/cGMP phosphodiesterase; ADSD2; cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; dJ416F21.1 (phosphodiesterase 10A); HSPDE10A; IOLOD; LINC00473; PDE10A19; phosphodiesterase 10A1 (PDE10A1); phosphodiesterase 10A7 (PDE10A7)
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDE10A using anti-PDE10A antibody (A02605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE10A antigen affinity purified polyclonal antibody (Catalog # A02605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE10A at approximately 100KD. The expected band size for PDE10A is at 100KD.
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