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Western Blot analysis of A549 cells using PDE10A Polyclonal Antibody
Western Blot analysis of A549 cells using PDE10A Polyclonal Antibody
Western Blot analysis of A549 cells using PDE10A Polyclonal Antibody

PDE10A Antibody

CSB-PA282556
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetPDE10A
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDE10A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10846
  • Target name
    PDE10A
  • Target description
    phosphodiesterase 10A
  • Target synonyms
    3',5' cAMP/cGMP phosphodiesterase; ADSD2; cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A; dJ416F21.1 (phosphodiesterase 10A); HSPDE10A; IOLOD; LINC00473; PDE10A19; phosphodiesterase 10A1 (PDE10A1); phosphodiesterase 10A7 (PDE10A7)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y233
  • Protein Name
    cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDE10A using anti-PDE10A antibody (A02605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE10A antigen affinity purified polyclonal antibody (Catalog # A02605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE10A at approximately 100KD. The expected band size for PDE10A is at 100KD.
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