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ProductsBack/PDE5A Rabbit Polyclonal Antibody
WB Suggested Anti-PDE5A Antibody Titration: 1.0 ug/ml Positive Control: Placenta
WB Suggested Anti-PDE5A Antibody Titration: 1.0 ug/ml Positive Control: Placenta
WB Suggested Anti-PDE5A Antibody Titration: 1.0 ug/ml Positive Control: Placenta

PDE5A Rabbit Polyclonal Antibody

TA358387
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetPDE5A
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PDE5A Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID8654
  • Target name
    PDE5A
  • Target description
    phosphodiesterase 5A
  • Target synonyms
    CGB-PDE; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; CN5A; PDE5; phosphodiesterase 5A, cGMP-specific; phosphodiesterase isozyme 5
  • Host
    Rabbit
  • Protein IDO76074
  • Protein Name
    cGMP-specific 3',5'-cyclic phosphodiesterase
  • Scientific Description
    PDE5A Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of PDE5A using anti-PDE5A antibody (A02490-2). PDE5A was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-PDE5A Antibody (A02490-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Lane 1: Human HT1080 cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with PDE5A Polyclonal Antibody, Unconjugated (bs-2349R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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