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PDGFC antibody [5F12]

GTX53022
GeneTex
ApplicationsNeutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetPDGFC
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Overview

  • Supplier
    GeneTex
  • Product Name
    PDGFC antibody [5F12]
  • Delivery Days Customer
    9
  • Applications
    Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5F12
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID56034
  • Target name
    PDGFC
  • Target description
    platelet derived growth factor C
  • Target synonyms
    FALLOTEIN, SCDGF, platelet-derived growth factor C, PDGF-C, VEGF-E, secretory growth factor-like protein, spinal cord-derived growth factor
  • Host
    Mouse
  • Isotype
    IgG2
  • Protein IDQ9NRA1
  • Protein Name
    Platelet-derived growth factor C
  • Scientific Description
    The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2010]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDGFC using anti-PDGFC antibody (A03092-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFC antigen affinity purified polyclonal antibody (Catalog # A03092-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFC at approximately 39 kDa. The expected band size for PDGFC is at 39 kDa.
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