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Western Blot analysis of HELA KB cells using PDGFR-beta Polyclonal Antibody
Western Blot analysis of HELA KB cells using PDGFR-beta Polyclonal Antibody
Western Blot analysis of HELA KB cells using PDGFR-beta Polyclonal Antibody

PDGFRB Antibody

CSB-PA003726
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDGFRB
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDGFRB Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5159
  • Target name
    PDGFRB
  • Target description
    platelet derived growth factor receptor beta
  • Target synonyms
    Activated tyrosine kinase PDGFRB; beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140B; IBGC4; IMF1; JTK12; KOGS; NDEL1-PDGFRB; PDGFR; PDGFR1; PDGFR-1; PDGF-R-beta; PDGFR-beta; PENTT; platelet-derived growth factor receptor 1; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor, beta polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09619
  • Protein Name
    Platelet-derived growth factor receptor beta
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDGFRB using anti-PDGFRB antibody (A00096-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SGC-7901 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRB antigen affinity purified polyclonal antibody (Catalog # A00096-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFRB at approximately 120KD. The expected band size for PDGFRB is at 124KD.
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