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ProductsBack/PDI / P4HB Antibody (clone 12, Preservative Free)

PDI / P4HB Antibody (clone 12, Preservative Free)

LS-C820128
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetP4HB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PDI / P4HB Antibody (clone 12, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA(Cap): This antibody will detect Human P4HB in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Applications
    ELISA
  • Applications Supplier
    ELISA ELISA(Cap): This antibody will detect Human P4HB in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    12
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5034
  • Target name
    P4HB
  • Target description
    prolyl 4-hydroxylase subunit beta
  • Target synonyms
    cellular thyroid hormone-binding protein; CLCRP1; collagen prolyl 4-hydroxylase beta; DSI; ERBA2L; GIT; glutathione-insulin transhydrogenase; P4Hbeta; p55; PDI; PDIA1; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; PROHB; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase family A, member 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P4HB using anti-P4HB antibody (A02335-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: monkey lung tissue lysates, Lane 8: monkey liver tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P4HB antigen affinity purified polyclonal antibody (Catalog # A02335-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P4HB at approximately 57KD. The expected band size for P4HB is at 57KD.
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