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ICC/IF analysis of methanol-fixed HeLa cells using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.
ICC/IF analysis of methanol-fixed HeLa cells using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.
ICC/IF analysis of methanol-fixed HeLa cells using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.

PDPK1 / PDK1 (phospho Ser241) antibody

GTX50093
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPDPK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PDPK1 / PDK1 (phospho Ser241) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. ICC/IF: 1:100-1:200. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5170
  • Target name
    PDPK1
  • Target description
    3-phosphoinositide dependent protein kinase 1
  • Target synonyms
    PDK1, PDPK2, PDPK2P, PRO0461, 3-phosphoinositide-dependent protein kinase 1, 3-phosphoinositide-dependent protein kinase 2 pseudogene, PkB kinase like gene 1, Putative 3-phosphoinositide-dependent protein kinase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15530
  • Protein Name
    3-phosphoinositide-dependent protein kinase 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cao W, Liu Y, Zhang R, et al. Homoharringtonine induces apoptosis and inhibits STAT3 via IL-6/JAK1/STAT3 signal pathway in Gefitinib-resistant lung cancer cells. Sci Rep. 2015,5:8477. doi: 10.1038/srep08477
    Read this paper

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Figure 1. Western blot analysis of PDPK1 using anti-PDPK1 antibody (PB9735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Lung Tissue Lysate, Lane 3: Mouse Liver Tissue Lysate, Lane 4: Mouse Lung Tissue Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: MCF-7 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody (Catalog # PB9735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPK1 at approximately 70KD. The expected band size for PDPK1 is at 63KD.
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