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ProductsBack/PDX1 Mouse Monoclonal Antibody [Clone ID: 5A5]
Western blot analysis using PDX1 antibody against Human PDX1 (AA: 39-283) recombinant protein. (Expected MW is 52 kDa)
Western blot analysis using PDX1 antibody against Human PDX1 (AA: 39-283) recombinant protein. (Expected MW is 52 kDa)
Western blot analysis using PDX1 antibody against Human PDX1 (AA: 39-283) recombinant protein. (Expected MW is 52 kDa)

PDX1 Mouse Monoclonal Antibody [Clone ID: 5A5]

AM06739PU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPDX1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PDX1 Mouse Monoclonal Antibody [Clone ID: 5A5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5A5
  • Gene ID3651
  • Target name
    PDX1
  • Target description
    pancreatic and duodenal homeobox 1
  • Target synonyms
    GSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1, pancreas/duodenum homeobox protein 1, IPF-1, IUF-1, glucose-sensitive factor, insulin promoter factor 1, homeodomain transcription factor, insulin upstream factor 1, islet/duodenum homeobox-1, pancreatic-duodenal homeobox factor 1, somatostatin transcription factor 1, somatostatin-transactivating factor 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP52945
  • Protein Name
    Pancreas/duodenum homeobox protein 1
  • Scientific Description
    PDX1 mouse monoclonal antibody, clone 5A5, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PDX1 using anti-PDX1 antibody (A00491-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDX1 antigen affinity purified polyclonal antibody (Catalog # A00491-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDX1 at approximately 42KD. The expected band size for PDX1 is at 42KD.
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