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PDXK Rabbit pAb

A6687
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDXK
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Overview

  • Supplier
    ABclonal
  • Product Name
    PDXK Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    WB,IF/ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8566
  • Target name
    PDXK
  • Target description
    pyridoxal kinase
  • Target synonyms
    C21orf124, C21orf97, HEL-S-1a, HMSN6C, PKH, PNK, PRED79, pyridoxal kinase, epididymis secretory sperm binding protein Li 1a, pyridoxal (pyridoxine, vitamin B6) kinase, pyridoxamine kinase, pyridoxine kinase, vitamin B6 kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00764
  • Protein Name
    Pyridoxal kinase
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Western blot All lanes: PDXK antibody at 5.63microg/ml Lane 1: Mouse liver tissue Lane 2: Hela whole cell lysate Lane 3: Mouse kidney tissue Lane 4: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 32, 27 kDa Observed band size: 36 kDa
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Figure 1. Western blot analysis of PDXK using anti-PDXK antibody (A06683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXK antigen affinity purified polyclonal antibody (Catalog # A06683-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDXK at approximately 35 kDa. The expected band size for PDXK is at 35,40 kDa.
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