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PE Anti-Human CD36 Antibody

RD21868F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD36
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    PE Anti-Human CD36 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5-271
  • Conjugate
    RPE
  • Gene ID948
  • Target name
    CD36
  • Target description
    CD36 molecule
  • Target synonyms
    BDPLT10; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); CHDS7; cluster determinant 36; FAT; fatty acid translocase; glycoprotein IIIb; GP3B; GP4; GPIIIB; GPIV; leukocyte differentiation antigen CD36; PAS IV; PAS-4 protein; PASIV; platelet glycoprotein 4; platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    CD36 is an 85 kD integral membrane glycoprotein, also known as GPIIIb, or GPIV. It is expressed on various epithelial and endothelial cells as well as erythrocytes, platelets, macrophages/monocytes and some macrophage-derived dendritic cells. CD36 functions as a scavenger receptor, binding thrombospondin, long chain fatty acids, oxidized LDL, collagen type I, IV, and V as well as apoptotic cells. The 5-271 antibody has been reported to be useful for flow cytometry.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD36 using anti-CD36 antibody (A01189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD36 antigen affinity purified polyclonal antibody (Catalog # A01189-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD36 at approximately 88 kDa. The expected band size for CD36 is at 53 kDa.
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