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IHC-P analysis of human colon cancer tissue using GTX04730 PEA3 antibody. Antigen retrieval : Heat mediated citrate buffer Dilution : 1:100
IHC-P analysis of human colon cancer tissue using GTX04730 PEA3 antibody. Antigen retrieval : Heat mediated citrate buffer Dilution : 1:100
IHC-P analysis of human colon cancer tissue using GTX04730 PEA3 antibody. Antigen retrieval : Heat mediated citrate buffer Dilution : 1:100

PEA3 antibody

GTX04730
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetETV4
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Overview

  • Supplier
    GeneTex
  • Product Name
    PEA3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2118
  • Target name
    ETV4
  • Target description
    ETS variant transcription factor 4
  • Target synonyms
    E1A-F, E1AF, PEA3, PEAS3, ETS translocation variant 4, EWS protein/E1A enhancer binding protein chimera, adenovirus E1A enhancer-binding protein, ets variant gene 4 (E1A enhancer-binding protein, E1AF), polyomavirus enhancer activator 3 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43268
  • Protein Name
    ETS translocation variant 4
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Pea3 using anti-Pea3 antibody (PB9699). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pea3 antigen affinity purified polyclonal antibody (Catalog # PB9699) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pea3 at approximately 68 kDa. The expected band size for Pea3 is at 54 kDa.
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