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PEA3 Antibody (YA4020, Mouse)

HY-P84323
MedChem Express
TargetETV4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PEA3 Antibody (YA4020, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2118
  • Target name
    ETV4
  • Target description
    ETS variant transcription factor 4
  • Target synonyms
    adenovirus E1A enhancer-binding protein; E1AF; E1A-F; ETS translocation variant 4; ETS variant 4; ets variant gene 4 (E1A enhancer-binding protein, E1AF); EWS protein/E1A enhancer binding protein chimera; PEA3; PEAS3; polyomavirus enhancer activator 3 homolog
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    PEA3 Antibody (YA4020) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ETV4. It can be applicated for WB, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Pea3 using anti-Pea3 antibody (PB9699). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pea3 antigen affinity purified polyclonal antibody (Catalog # PB9699) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pea3 at approximately 68 kDa. The expected band size for Pea3 is at 54 kDa.
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