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PECAM-1 / CD31 Antibody (clone 390, PE, Cy7)

LS-C812097
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetPECAM1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PECAM-1 / CD31 Antibody (clone 390, PE, Cy7)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 microL of antibody per test (million cells in 100 microl staining volume or per 100 microl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    390
  • Conjugate
    Cy7, RPE
  • Estimated Purity
    ...
  • Gene ID5175
  • Target name
    PECAM1
  • Target description
    platelet and endothelial cell adhesion molecule 1
  • Target synonyms
    CD31; CD31 antigen; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1
  • Host
    Rat
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31/PECAM1 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31/PECAM1 at approximately 90 kDa, 130 kDa. The expected band size for CD31/PECAM1 is at 82 kDa.
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