Bio-Connect

PE/Cyanine5 Anti-Mouse CD19 Antibody

RD20008F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd19
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Reddot Biotech
  • Product Name
    PE/Cyanine5 Anti-Mouse CD19 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D3
  • Conjugate
    Tricolor
  • Gene ID12478
  • Target name
    Cd19
  • Target description
    CD19 antigen
  • Target synonyms
    AW495831; B-lymphocyte antigen CD19; differentiation antigen CD19
  • Host
    Rat
  • Isotype
    IgG2a
  • Scientific Description
    CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsFlow Cytometry
TargetCd19
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry
TargetCd19
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded mouse embryo tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD19 Polyclonal Antibody, Unconjugated (bs-0079R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Product group Antibodies
References
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCd19
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCd19
  • SizePrice
Product group Antibodies
Assay Genie
ApplicationsFlow Cytometry
TargetCd19
  • SizePrice
Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: human K562 whole cell lysates, Lane 6: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
Product group Antibodies
Boster Bio
Anti-CD19 Antibody Picoband(r)A00154-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetCd19
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
TargetCd19
  • SizePrice