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PEG3 Antibody

ORB126237
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPEG3
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Overview

  • Supplier
    Biorbyt
  • Product Name
    PEG3 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB: 1:500-1:3000, ELISA: 1:10000 WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5178
  • Target name
    PEG3
  • Target description
    paternally expressed 3
  • Target synonyms
    PW1, ZKSCAN22, ZNF904, ZSCAN24, paternally-expressed gene 3 protein, Kruppel-type zinc finger protein, zinc finger and SCAN domain-containing protein 24
  • Host
    Rabbit
  • Protein IDQ9GZU2
  • Protein Name
    Paternally-expressed gene 3 protein
  • Scientific Description
    Rabbit Polyclonal to PEG3.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PEG3 Polyclonal Antibody(bs-1870R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of PEG3 using anti-PEG3 antibody (A02541-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEG3 antigen affinity purified polyclonal antibody (Catalog # A02541-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEG3 at approximately 200 kDa. The expected band size for PEG3 is at 181 kDa.
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