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PEPCK (E-1)

SC-271204
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPCK1
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    PEPCK (E-1)
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5105
  • Target name
    PCK1
  • Target description
    phosphoenolpyruvate carboxykinase 1
  • Target synonyms
    PCKDC, PEPCK-C, PEPCK1, PEPCKC, phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEP carboxykinase, phosphoenolpyruvate carboxykinase 1 (soluble), phosphoenolpyruvate carboxykinase, cytosolic, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase, serine-protein kinase PCK1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCK1/PEPC using anti-PCK1/PEPC antibody (A02022-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK1/PEPC antigen affinity purified polyclonal antibody (Catalog # A02022-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK1/PEPC at approximately 69KD. The expected band size for PCK1/PEPC is at 69KD.
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