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PER3 Antibody (C-Terminus)

LS-C17256
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetPER3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PER3 Antibody (C-Terminus)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Suitable for use in ELISA, Western Blot, Immunohistochemistry. Western Blot: 1-10 ug/ml using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well. Immunohistochemistry: 2-20 ug/ml. Frozen, free floating sections.. ELISA (1:10000 - 1:100000), IHC, IHC-Fr, WB (1 - 10 µg/ml) Suitable for use in ELISA, Western Blot, Immunohistochemistry. Western Blot: 1-10 ug/ml using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well. Immunohistochemistry: 2-20 ug/ml. Frozen, free floating sections.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8863
  • Target name
    PER3
  • Target description
    period circadian regulator 3
  • Target synonyms
    cell growth-inhibiting gene 13 protein; circadian clock protein PERIOD 3; FASPS3; GIG13; period circadian clock 3; period circadian protein 3; period circadian protein homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PER3 using anti-PER3 antibody (A01835-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human MCF-7 cell lysate, Lane 3: human A549 cell lysate, Lane 4: human HepG2 cell lysate, Lane 5: human COLO-320 cell lysate, Lane 6: rat heart tissue lysate, Lane 7: mouse skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PER3 antigen affinity purified polyclonal antibody (Catalog # A01835-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PER3 at approximately 160KD. The expected band size for PER3 is at 132KD.
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