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Perilipin A Monoclonal Antibody

CAB4758
Assay Genie
TargetPLIN1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Perilipin A Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5346
  • Target name
    PLIN1
  • Target description
    perilipin 1
  • Target synonyms
    FPLD4, PERI, PLIN, perilipin-1, lipid droplet-associated protein
  • Host
    Rabbit
  • Protein IDO60240
  • Protein Name
    Perilipin-1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Perilipin A using anti-Perilipin A antibody (A03918-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perilipin A antigen affinity purified polyclonal antibody (Catalog # A03918-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Perilipin A at approximately 62KD. The expected band size for Perilipin A is at 56KD.
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