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ProductsBack/PERK antibody [5G5]
WB analysis of HEK293 (1) and PERK(AA: 929-1116)-hIgGFc transfected HEK293 (2) cell lysate using GTX82759 PERK antibody [5G5].
WB analysis of HEK293 (1) and PERK(AA: 929-1116)-hIgGFc transfected HEK293 (2) cell lysate using GTX82759 PERK antibody [5G5].
WB analysis of HEK293 (1) and PERK(AA: 929-1116)-hIgGFc transfected HEK293 (2) cell lysate using GTX82759 PERK antibody [5G5].

PERK antibody [5G5]

GTX82759
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetEIF2AK3
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Overview

  • Supplier
    GeneTex
  • Product Name
    PERK antibody [5G5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5G5
  • Conjugate
    Unconjugated
  • Gene ID9451
  • Target name
    EIF2AK3
  • Target description
    eukaryotic translation initiation factor 2 alpha kinase 3
  • Target synonyms
    PEK, PERK, WRS, eukaryotic translation initiation factor 2-alpha kinase 3, PKR-like ER kinase, PRKR-like endoplasmic reticulum kinase, pancreatic EIF2-alpha kinase, pancreatic eIF-2alpha kinase, protein tyrosine kinase EIF2AK3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9NZJ5
  • Protein Name
    Eukaryotic translation initiation factor 2-alpha kinase 3
  • Scientific Description
    The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PERK using anti-PERK antibody (A01992-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: Human A431 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PERK antigen affinity purified polyclonal antibody (Catalog # A01992-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PERK at approximately 140KD. The expected band size for PERK is at 125KD.
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