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PFAS Antibody (Biotin)

LS-C678994
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetPFAS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PFAS Antibody (Biotin)
  • Delivery Days Customer
    14
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID5198
  • Target name
    PFAS
  • Target description
    phosphoribosylformylglycinamidine synthase
  • Target synonyms
    FGAM synthase; FGAMS; FGAR amidotransferase; FGARAT; FGAR-AT; formylglycinamide ribonucleotide amidotransferase; formylglycinamide ribotide amidotransferase; formylglycinamide ribotide synthetase; GATD8; IKZF1/PFAS fusion; phosphoribosylformylglycinamidine synthase; PURL
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PFAS using anti-PFAS antibody (A03795-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U937 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat brain tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 12: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFAS antigen affinity purified polyclonal antibody (Catalog # A03795-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFAS at approximately 145KD. The expected band size for PFAS is at 145KD.
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