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WB analysis of various samples using GTX04818 PFAS antibody. Loading : 25 microg Dilution : 1:3000
WB analysis of various samples using GTX04818 PFAS antibody. Loading : 25 microg Dilution : 1:3000
WB analysis of various samples using GTX04818 PFAS antibody. Loading : 25 microg Dilution : 1:3000

PFAS antibody

GTX04818
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPFAS
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Overview

  • Supplier
    GeneTex
  • Product Name
    PFAS antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5198
  • Target name
    PFAS
  • Target description
    phosphoribosylformylglycinamidine synthase
  • Target synonyms
    FGAM synthase; FGAMS; FGAR amidotransferase; FGARAT; FGAR-AT; formylglycinamide ribonucleotide amidotransferase; formylglycinamide ribotide amidotransferase; formylglycinamide ribotide synthetase; GATD8; IKZF1/PFAS fusion; phosphoribosylformylglycinamidine synthase; PURL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15067
  • Protein Name
    Phosphoribosylformylglycinamidine synthase
  • Scientific Description
    Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PFAS using anti-PFAS antibody (A03795-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U937 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat brain tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 12: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFAS antigen affinity purified polyclonal antibody (Catalog # A03795-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFAS at approximately 145KD. The expected band size for PFAS is at 145KD.
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