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PFKFB2 Antibody

LS-C410845
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPFKFB2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PFKFB2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 54kDa/58kDa, while the observed MW by Western blot was 54kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 54kDa/58kDa, while the observed MW by Western blot was 54kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5208
  • Target name
    PFKFB2
  • Target description
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
  • Target synonyms
    PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme, 6PF-2-K/Fru-2,6-P2ase 2, PFK/FBPase 2, PFKFB, cardiac, fructose-2,6-bisphosphatase, cardiac isozyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PFKFB2 using anti-PFKFB2 antibody (A05977). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse heart tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKFB2 antigen affinity purified polyclonal antibody (Catalog # A05977) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKFB2 at approximately 58KD. The expected band size for PFKFB2 is at 58KD.
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