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WB analysis of various sample lysates using GTX55744 PGD antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX55744 PGD antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX55744 PGD antibody. Dilution : 1:1000 Loading : 25microg per lane

PGD antibody

GTX55744
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPGD
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Overview

  • Supplier
    GeneTex
  • Product Name
    PGD antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5226
  • Target name
    PGD
  • Target description
    phosphogluconate dehydrogenase
  • Target synonyms
    6PGD; 6-phosphogluconate dehydrogenase, decarboxylating
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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WB analysis of various samples using GTX57669 PGD antibody. Lane 1 : HeLa whole cell lysate Lane 2 : MCF-7 whole cell lysate Lane 3 : 293T whole cell lysate Lane 4 : A549 whole cell lysate Lane 5 : Jurkat whole cell lysate Loading : 40 microg Dilution : 1:1000
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Western blot All lanes: PGD antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 54, 52 kDa Observed band size: 54 kDa
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Figure 1. Western blot analysis of PGD using anti-PGD antibody (A01623-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGD antigen affinity purified polyclonal antibody (Catalog # A01623-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGD at approximately 48 kDa. The expected band size for PGD is at 54,48 kDa.
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