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ProductsBack/PGR Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human breast caricnoma using PR Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded human breast caricnoma using PR Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded human breast caricnoma using PR Monoclonal Antibody.

PGR Monoclonal Antibody

CSB-MA080142
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPGR
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Overview

  • Supplier
    Cusabio
  • Product Name
    PGR Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5241
  • Target name
    PGR
  • Target description
    progesterone receptor
  • Target synonyms
    NR3C3; nuclear receptor subfamily 3 group C member 3; PR; progesterone receptor
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP06401
  • Protein Name
    Progesterone receptor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with progesterone receptor Antibody(bs-0111R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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