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WB analysis of human kidney (A), liver (B) and testis (C) lysate (35μg protein in RIPA buffer) using GTX89362 PGRMC1 antibody, C-term. Dilution : 0.1μg/ml
WB analysis of human kidney (A), liver (B) and testis (C) lysate (35μg protein in RIPA buffer) using GTX89362 PGRMC1 antibody, C-term. Dilution : 0.1μg/ml
WB analysis of human kidney (A), liver (B) and testis (C) lysate (35μg protein in RIPA buffer) using GTX89362 PGRMC1 antibody, C-term. Dilution : 0.1μg/ml

PGRMC1 antibody, C-term

GTX89362
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPGRMC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PGRMC1 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. IHC-P: 5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10857
  • Target name
    PGRMC1
  • Target description
    progesterone receptor membrane component 1
  • Target synonyms
    Dap1, HPR6.6, IZA, MPR, membrane-associated progesterone receptor component 1, progesterone binding protein
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDO00264
  • Protein Name
    Membrane-associated progesterone receptor component 1
  • Scientific Description
    This gene encodes a putative membrane-associated progesterone steroid receptor. The protein is expressed predominantly in the liver and kidney. [provided by RefSeq, Mar 2010]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Progesterone receptor membrane associated component 1 enhances obesity progression in mice by facilitating lipid accumulation in adipocytes. Furuhata R et al., 2020 Sep 4, Commun Biol
    Read this paper

Datasheet

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Figure 1. Western blot analysis of PGRMC1 using anti-PGRMC1 antibody (PB9775). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: Mouse Liver Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug, Lane 5: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGRMC1 antigen affinity purified polyclonal antibody (Catalog # PB9775) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGRMC1 at approximately 26 kDa. The expected band size for PGRMC1 is at 26 kDa.
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