More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PHB Polyclonal Antibody
Western Blot analysis of 293T and A549 cell,Human liver cancer tissue and PC3 cell ,Human placenta tissue,hela cell and Mouse heart tissue using PHB Polyclonal Antibody at dilution of 1:350
Western Blot analysis of 293T and A549 cell,Human liver cancer tissue and PC3 cell ,Human placenta tissue,hela cell and Mouse heart tissue using PHB Polyclonal Antibody at dilution of 1:350
Western Blot analysis of 293T and A549 cell,Human liver cancer tissue and PC3 cell ,Human placenta tissue,hela cell and Mouse heart tissue using PHB Polyclonal Antibody at dilution of 1:350

PHB Polyclonal Antibody

E-AB-10541
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetPHB1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Elabscience
  • Product Name
    PHB Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5245
  • Target name
    PHB1
  • Target description
    prohibitin 1
  • Target synonyms
    BAP32, HEL-215, HEL-S-54e, PHB, prohibitin 1, epididymis luminal protein 215, epididymis secretory sperm binding protein Li 54e, prohibitin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35232
  • Protein Name
    Prohibitin 1
  • Scientific Description
    This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3 UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
Prohibitin AntibodyCAB0056
ReactivityHuman
TargetPHB1
  • SizePrice
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA014587(PHB Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
Product group Antibodies
Cusabio
PHB AntibodyCSB-PA014587
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPHB1
  • SizePrice
Figure 1. Western blot analysis of Prohibitin/PHB using anti-Prohibitin/PHB antibody (A01178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hek293 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HT1080 whole cell lysates, Lane 6: human Sw620 whole cell lysates, Lane 7: human U87 whole cell lysates, Lane 8: human A549 whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: rat PC-12 whole cell lysates, Lane 13: mouse brain tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse liver tissue lysates, Lane 16: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prohibitin/PHB antigen affinity purified polyclonal antibody (Catalog # A01178-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prohibitin/PHB at approximately 28-30KD. The expected band size for Prohibitin/PHB is at 28-30KD.
Product group Antibodies
Boster Bio
Anti-Prohibitin/PHB Antibody Picoband(r)A01178-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPHB1
  • SizePrice
Anti-PHB Antibody
Product group Antibodies
Antibodies.com
Anti-PHB AntibodyA29414
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-PHB AntibodyHPA003280
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPHB1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PHB / Prohibitin AntibodyLS-C400968
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPHB1
  • SizePrice
Product group Antibodies
Biomatik
PHB Polyclonal AntibodyCAC13157
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetPHB1
  • SizePrice
Mouse heart lysates probed with Prohibitin Polyclonal Antibody, Unconjugated (bs-1449R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
Prohibitin Polyclonal AntibodyBS-1449R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityCanine, Equine, Human, Mouse, Rat
TargetPHB1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
Prohibitin antibodyGTX101105
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetPHB1
  • SizePrice
Product group Antibodies
Biosynth
PHB antibody70R-19252
  • SizePrice